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amaxa basic nucleofector kit primary mammalian fibroblasts  (Lonza)


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    Lonza amaxa basic nucleofector kit primary mammalian fibroblasts
    Amaxa Basic Nucleofector Kit Primary Mammalian Fibroblasts, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/amaxa+basic+nucleofector+kit/4d+nucleofector/pm39129390-71-46-49
    Average 90 stars, based on 1 article reviews
    amaxa basic nucleofector kit primary mammalian fibroblasts - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia
    Article Snippet: As control, a negative-control siRNA sequence was employed (#sc-37007, Santa Cruz Biotechnology, Heidelberg, Germany). .. Cells were transfected with siRNA using Amaxa Basic Nucleofector Kit (#VPI-1002, Lonza, Cologne, Germany). ..

    Article Title: Multiple Mechanisms Driving F-actin-Dependent Transport of Organelles to and From Secretory Sites in Bovine Chromaffin Cells
    Article Snippet: .. Single and dual transfection of isolated chromaffin cells using the Amaxa basic nucleofector kit (Lonza, red VVPG-1001) served to visualize the F-actin cytoskeleton and chromaffin granules in living primary mammalian neurons (Program 0–005, Amaxa GmbH. ..

    Article Title: FcRn is not the receptor mediating the transfer of serum IgG to colostrum in pigs.
    Article Snippet: Cuncun Ke, Yonghe Ma, Dengke Pan and Zihui Wan authors contributed equally to this work.. Abbreviations: FcRn, neonatal Fc receptor; PFFs, porcine fetal fibroblasts; SCNT, somatic cell nuclear transfer; Indel, insertion/deletion; WT, wild type; KO, knockout; In1A, a single adenine insertion; OT, offtarget.. 1State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing, China 2Joint National Laboratory for Antibody Drug Engineering, Henan International Union Lab of Antibody Medicine, Henan University School of Medicine, Kaifeng, China 3Clinical Immunology Translational Medicine Key Laboratory of Sichuan Province, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China 4Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China 5Henan Engineering Laboratory for Mammary Bioreactor, School of Life Science, Henan University, Kaifeng, China

    Article Title: Forkhead-box transcription factor 1 affects the apoptosis of natural regulatory T cells by controlling Aven expression
    Article Snippet: .. Using the Nucleofector Program U-25, 2 mg of plasmid was transfected into 1 × 10 6 cells using the Amaxa Basic Nucleofector kit (Lonza, Switzerland), according to the manufacturer’s protocol. .. Total RNA was extracted in Trizol LS reagent (Takara, Japan) and reverse-transcribed using a PrimeScript RT reagent kit (Takara).

    Article Title: Pericyte Implantation in the Brain Enhances Cerebral Blood Flow and Reduces Amyloid-β Pathology in Amyloid Model Mice
    Article Snippet: For GFP transfection, the pmax GFP vector provided in Amaxa Basic Nucleofector Kit (Lonza; Cologne, Germany) was used. .. LRP1-siRNA (300 nM) or GFP plasmid vector (2 μg) was transfected into C3H/10T1/2 cell-derived pericytes by nucleofection using the Amaxa Basic Nucleofector Kit (Lonza) according to the manufacturer’s instructions (program A-033). ..

    Article Title: Histone Deacetylase 1 Regulates the Expression of Progesterone Receptor A During Human Parturition by Occupying the Progesterone Receptor A Promoter.
    Article Snippet: The functional withdrawal of progesterone is mediated by the enhanced expression ratio of the 2 progesterone receptor (PR) isoforms, PRA and PRB, and causes the pregnant human myometrium to switch from a quiet state to a state of excitation– contraction and subsequent laboring.. However, the precise mechanisms responsible for alterations in PRA and PRB expression during human parturition have yet to be resolved.. In the present study, we report that PRA expression was increased in myometrium samples during labor (P < .001), concomitant with reduced expression of histone deacetylase 1 (HDAC1; P < .01).

    Isolation:

    Article Title: Multiple Mechanisms Driving F-actin-Dependent Transport of Organelles to and From Secretory Sites in Bovine Chromaffin Cells
    Article Snippet: .. Single and dual transfection of isolated chromaffin cells using the Amaxa basic nucleofector kit (Lonza, red VVPG-1001) served to visualize the F-actin cytoskeleton and chromaffin granules in living primary mammalian neurons (Program 0–005, Amaxa GmbH. ..

    Plasmid Preparation:

    Article Title: Forkhead-box transcription factor 1 affects the apoptosis of natural regulatory T cells by controlling Aven expression
    Article Snippet: .. Using the Nucleofector Program U-25, 2 mg of plasmid was transfected into 1 × 10 6 cells using the Amaxa Basic Nucleofector kit (Lonza, Switzerland), according to the manufacturer’s protocol. .. Total RNA was extracted in Trizol LS reagent (Takara, Japan) and reverse-transcribed using a PrimeScript RT reagent kit (Takara).

    Article Title: Pericyte Implantation in the Brain Enhances Cerebral Blood Flow and Reduces Amyloid-β Pathology in Amyloid Model Mice
    Article Snippet: For GFP transfection, the pmax GFP vector provided in Amaxa Basic Nucleofector Kit (Lonza; Cologne, Germany) was used. .. LRP1-siRNA (300 nM) or GFP plasmid vector (2 μg) was transfected into C3H/10T1/2 cell-derived pericytes by nucleofection using the Amaxa Basic Nucleofector Kit (Lonza) according to the manufacturer’s instructions (program A-033). ..

    Article Title: Histone Deacetylase 1 Regulates the Expression of Progesterone Receptor A During Human Parturition by Occupying the Progesterone Receptor A Promoter.
    Article Snippet: The functional withdrawal of progesterone is mediated by the enhanced expression ratio of the 2 progesterone receptor (PR) isoforms, PRA and PRB, and causes the pregnant human myometrium to switch from a quiet state to a state of excitation– contraction and subsequent laboring.. However, the precise mechanisms responsible for alterations in PRA and PRB expression during human parturition have yet to be resolved.. In the present study, we report that PRA expression was increased in myometrium samples during labor (P < .001), concomitant with reduced expression of histone deacetylase 1 (HDAC1; P < .01).

    Article Title: Pericyte Implantation in the Brain Enhances Cerebral Blood Flow and Reduces Amyloid-β Pathology in Amyloid Model Mice
    Article Snippet: Non-targeting siRNA was also purchased from Dharmacon (GE Healthcare) and used as the control. .. For GFP transfection, the pmax GFP vector provided in Amaxa Basic Nucleofector Kit (Lonza; Cologne, Germany) was used. .. LRP1-siRNA (300 nM) or GFP plasmid vector (2 μg) was transfected into C3H/10T1/2 cell-derived pericytes by nucleofection using the Amaxa Basic Nucleofector Kit (Lonza) according to the manufacturer’s instructions (program A-033).



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    Image Search Results


    Journal: Life Science Alliance

    Article Title: An isoform-specific function of Cdc42 in regulating mammalian Exo70 during axon formation

    doi: 10.26508/lsa.202201722

    Figure Lengend Snippet:

    Article Snippet: For analysis of exocytosis at 1 d.i.v., primary hippocampal neurons were transfected by nucleofection using Nucleofector II (Amaxa) and the Amaxa Basic Neuron SCN Nucleofector kit (Lonza).

    Techniques: Recombinant, Sequencing, Software, Blocking Assay, Expressing, Plasmid Preparation